Characteristic Pigmentation Motility NaCl range (M) NaCl optimum (M) Minimum Mg2+ Temp range (°C) Temp optimum (°C) pH range Generation time (hr) Oxidase test H2S formation from thiosulfate Hydrolysis of Gelatin Hydrolysis of Casein Hydrolysis of Starch Hydrolysis of Tween 80 Acid production on Mannose Acid production on Galactose Acid production on Xylose Acid production on Sucrose Rifampicin Resistant Bactitracin Resistant DNA G+C content (%mol) DNA-DNA reassociation with SA5 DNA-DNA reassociation with PA12 SA5 red rotating 1.7-5.1 2.6-3.4 0.2 30-55 53 7.0-9.0 0.53 (at 53°C) ± - + + + + - - - + + + 61.4 100 30 PA12 pink-red - 1.7-5.1 2.6-3.4 0.2 23-55 42-53 6.0-10 0.96 (at 48°C) - - + + - - - - - + + + 60.8 18 100 Hfx. Mediterranei pink + 1.3-4.7 2.9 0.02 25-45 35-37 ND 1.2 + - + + + + + ND + + - - 60 20 (19) 14 (15) Hfx. volcanii red-orange rotating 1.0-4.5 1.7-2.5 0.02 ND 45 ND 1.83 + + - - - - - + + + ND ND 63.4 29 (18) 29 (17) Hfx. Denitrificans pink + 1.8-5.1 4.3 ND 10.0-40.0 37 5.0-9.0 ND + + - - - + ND - + - ND ND 64.5 20 (27) 22 (16) Hfx. Gibbonsii orange-red + 1.5-5.2 2.5-4.3 0.2 25-55 35-40 5.0-8.0 ND + + + + - + + + + + - - 61.8 25 (23) 30 (20) Hfx. Sulfurifontis salmon pink - 1.0-5.2 2.1-2.6 0.001 18-50 32-37 4.5-9.0 ND + + + - - + - + + + - + 60.5 25 (33) 19 (21) Hfx. Lucentense Pink + 1.8-5.1 4.3 ND 10.0-40.0 37 5.0-9 ND + + - - - + ND - + - ND ND 64.5 20 (27) 22 (16) Hfx. Alexandrinus Red - 1.7-5.2 4.3 0.33 20-55 37 5.5-7.5 ND + + + - - + - - + + + - 59.5 24 (19) 24 (32) Hfx. Prahovense Beige- orange - 2.5-5.2 3.5 ND 23-51 38-48 6.0-8.5 ND + + - - + + ND - - - - + 63.7 20 (25) 21 (15) Hfx. Larsenii Orange-red + 1.0-4.8 2.2-3.4 0.005 25-55 42-45 6.0-8.5 ND + + + - + + - - - weakly + - - 62.2 22 (19) 24 (22) Characteristic Pigmentation Motility NaCl range (M) NaCl optimum (M) Minimum Mg2+ Temp range (°C) Temp optimum (°C) pH range Generation time (hr) Oxidase test H2S formation from thiosulfate Hydrolysis of Gelatin Hydrolysis of Casein Hydrolysis of Starch Hydrolysis of Tween 80 Acid production on Mannose Acid production on Galactose Acid production on Xylose Acid production on Sucrose Rifampicin Resistant Bactitracin Resistant DNA G+C content (%mol) DNA-DNA reassociation with SA5 DNA-DNA reassociation with PA12 SA5 red rotating 1.7-5.1 2.6-3.4 0.2 30-55 53 7.0-9.0 0.53 (at 53°C) ± - + + + + - - - + + + 61.4 100 30 PA12 pink-red - 1.7-5.1 2.6-3.4 0.2 23-55 42-53 6.0-10 0.96 (at 48°C) - - + + - - - - - + + + 60.8 18 100 Hfx. Mediterranei pink + 1.3-4.7 2.9 0.02 25-45 35-37 ND 1.2 + - + + + + + ND + + - - 60 20 (19) 14 (15) Hfx. volcanii red-orange rotating 1.0-4.5 1.7-2.5 0.02 ND 45 ND 1.83 + + - - - - - + + + ND ND 63.4 29 (18) 29 (17) Hfx. Denitrificans pink + 1.8-5.1 4.3 ND 10.0-40.0 37 5.0-9.0 ND + + - - - + ND - + - ND ND 64.5 20 (27) 22 (16) Hfx. Gibbonsii orange-red + 1.5-5.2 2.5-4.3 0.2 25-55 35-40 5.0-8.0 ND + + + + - + + + + + - - 61.8 25 (23) 30 (20) Hfx. Sulfurifontis salmon pink - 1.0-5.2 2.1-2.6 0.001 18-50 32-37 4.5-9.0 ND + + + - - + - + + + - + 60.5 25 (33) 19 (21) Hfx. Lucentense Pink + 1.8-5.1 4.3 ND 10.0-40.0 37 5.0-9 ND + + - - - + ND - + - ND ND 64.5 20 (27) 22 (16) Hfx. Alexandrinus Red - 1.7-5.2 4.3 0.33 20-55 37 5.5-7.5 ND + + + - - + - - + + + - 59.5 24 (19) 24 (32) Hfx. Prahovense Beige- orange - 2.5-5.2 3.5 ND 23-51 38-48 6.0-8.5 ND + + - - + + ND - - - - + 63.7 20 (25) 21 (15) Hfx. Larsenii Orange-red + 1.0-4.8 2.2-3.4 0.005 25-55 42-45 6.0-8.5 ND + + + - + + - - - weakly + - - 62.2 22 (19) 24 (22)