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Jeremy's Lab Blog

Contamination of sequencing data (Pt. 2)

It is amazing how easily the processing of samples can lead to contamination of data. Something like 22% of sequenced genomes contain AluY elements from the human genome. As noted in the following posting from The Scientist, this alarming discovery could also be indicative of contamination of sequenced genomes by DNA from other sources, such as the commonly used E. coli, which could be problematic when working with other bacterial genomes. This possibility could have grave consequences when it comes to evaluating horizontal gene transfer.

http://www.the-scientist.com/news/display/57990/

http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016410

Though, it should be noted that as per the article in The Scientist, this is only applicable to female scientists ("But probably the most common contaminant is the scientist herself." from paragraph 4).

 

Extreme caution is needed when sequencing

One of my friends from U of I sent me an interesting discussion of a recent paper with a potentially fatal error.....Note the article's editor...hehe. Anyway, the paper talks about a horizontally transferred gene from the human genome to the genome of the intracellular pathogen, Neisseria gonorrhoeae (http://mbio.asm.org/content/2/1/e00005-11.full).

The following blog has an interesting description of a more plausible reason for the published finding:

http://pathogenomics.bham.ac.uk/blog/2011/02/human-dna-in-bacterial-genomes-yes-no-maybe/

Reminds me of the story about Shewanella and Burkholderia being the clearly dominating organisms in one of the Sargasso Sea metagenomes...(http://www.nature.com/nrmicro/journal/v3/n6/pdf/nrmicro1158.pdf)